RASSF1A Suppresses the c-Jun-NH2-Kinase Pathway and Inhibits Cell Cycle Progression
YM Whang, YH Kim, JS Kim, YD Yoo - Cancer research, 2005 - AACR
YM Whang, YH Kim, JS Kim, YD Yoo
Cancer research, 2005•AACRSome oncogenes, such as activated Ras, cause the malignant transformation of lung cells. c-
Jun-NH2-kinase (JNK) activation is essential for the oncogenic function of these cells. In this
study, we show that RASSF1A inhibits the growth of lung cancer cells by blocking the JNK
pathway. The exogenous expression of RASSF1A suppressed JNK phosphorylation, and
cells stably transfected with RASSF1A showed reduced JNK and c-Jun phosphorylation and
Cyclin D1 down-regulation. An in vitro kinase assay showed that the exogenous expression …
Jun-NH2-kinase (JNK) activation is essential for the oncogenic function of these cells. In this
study, we show that RASSF1A inhibits the growth of lung cancer cells by blocking the JNK
pathway. The exogenous expression of RASSF1A suppressed JNK phosphorylation, and
cells stably transfected with RASSF1A showed reduced JNK and c-Jun phosphorylation and
Cyclin D1 down-regulation. An in vitro kinase assay showed that the exogenous expression …
Abstract
Some oncogenes, such as activated Ras, cause the malignant transformation of lung cells. c-Jun-NH2-kinase (JNK) activation is essential for the oncogenic function of these cells. In this study, we show that RASSF1A inhibits the growth of lung cancer cells by blocking the JNK pathway. The exogenous expression of RASSF1A suppressed JNK phosphorylation, and cells stably transfected with RASSF1A showed reduced JNK and c-Jun phosphorylation and Cyclin D1 down-regulation. An in vitro kinase assay showed that the exogenous expression of RASSF1A inhibited JNK activity and that JNK activity suppression due to ectopically expressed RASSF1A was revived by RASSF1A siRNA treatment. Based on our data, we suggest that RASSF1A exerts a tumor-suppressing effect by blocking oncogene-mediated JNK activation in lung cells.
AACR
