[HTML][HTML] Phosphorylation-induced conformational changes in Rap1b: allosteric effects on switch domains and effector loop
MM Edreira, S Li, D Hochbaum, S Wong… - Journal of Biological …, 2009 - ASBMB
Rap1b has been implicated in the transduction of the cAMP mitogenic response. Agonists
that increase intracellular cAMP rapidly activate (ie GTP binding) and phosphorylate Rap1b
on Ser 179 at its C terminus. cAMP-dependent protein kinase (PKA)-mediated
phosphorylation of Rap1b is required for cAMP-dependent mitogenesis, tumorigenesis, and
inhibition of AKT activity. However, the role of phosphorylation still remains unknown. In this
study, we utilized amide hydrogen/deuterium exchange mass spectroscopy (DXMS) to …
that increase intracellular cAMP rapidly activate (ie GTP binding) and phosphorylate Rap1b
on Ser 179 at its C terminus. cAMP-dependent protein kinase (PKA)-mediated
phosphorylation of Rap1b is required for cAMP-dependent mitogenesis, tumorigenesis, and
inhibition of AKT activity. However, the role of phosphorylation still remains unknown. In this
study, we utilized amide hydrogen/deuterium exchange mass spectroscopy (DXMS) to …