Permanent canine kidney (MDCK) cells for isolation and plaque assay of influenza B viruses
K Tobita - Medical microbiology and immunology, 1975 - Springer
K Tobita
Medical microbiology and immunology, 1975•SpringerA wide range of influenza B virus strains with various passage histories uniformly formed
well-defined clear plaques with high efficiency in cultures of an established line of canine
kidney cells (MDCK). PFU titers of the viruses assayed in MDCK exceeded the titers
assayed in ovo. With recently isolated strains such as B/Hong Kong/5/72 and Gifu/2/73, the
PFU/EID 50 ratios were as high as 100 to 400. MDCK cells have been successfully
employed for primary isolation of influenza B viruses from throat washings of patients by …
well-defined clear plaques with high efficiency in cultures of an established line of canine
kidney cells (MDCK). PFU titers of the viruses assayed in MDCK exceeded the titers
assayed in ovo. With recently isolated strains such as B/Hong Kong/5/72 and Gifu/2/73, the
PFU/EID 50 ratios were as high as 100 to 400. MDCK cells have been successfully
employed for primary isolation of influenza B viruses from throat washings of patients by …
Abstract
A wide range of influenza B virus strains with various passage histories uniformly formed well-defined clear plaques with high efficiency in cultures of an established line of canine kidney cells (MDCK). PFU titers of the viruses assayed in MDCK exceeded the titers assayedin ovo. With recently isolated strains such as B/Hong Kong/5/72 and Gifu/2/73, the PFU/EID50 ratios were as high as 100 to 400. MDCK cells have been successfully employed for primary isolation of influenza B viruses from throat washings of patients by direct plaquing.
Springer